In the process of liquid chromatography analysis, ghost peaks are often encountered, which interfere with the separation or quantification of drug analysis. These ghost peaks are generally introduced by impurities present in the mobile phase or added buffer salts or acid-base substances, which can easily interfere with the analysis during gradient elution analysis. The common solution is for engineers to thoroughly inspect the mobile phases in the system, such as methanol, water, buffer salts, acid and alkali, as well as instrument pipelines, which is time-consuming and labor-intensive.
Aijer Fenomi launched the Ghost Hunter Ghost Peak Capture Column with great enthusiasm to address this challenge and provide chromatographic analysis engineers with a powerful tool that can effectively remove ghost peak interference. Thus eliminating the interference of ghost peaks on experimental method validation and analysis of trace and micro substances, significantly reducing experimental time, and improving work efficiency.
The material for the ghost peak capture column is selected as an adsorbent material with a special structure and high surface activity, which can effectively adsorb various non-polar and polar organic impurities, thereby eliminating impurities in the mobile phase, reducing the occurrence of ghost peaks, and also extending the life of the chromatographic column and instrument.
Product Features
Continuously capture and remove ghost peaks
Resistant to water and organic solvents
Extend the lifespan of chromatography columns and instruments
Reduce the workload of data analysis
Easy to install and use
Simply installing a ghost peak capture column after the liquid-phase mobile phase mixer can easily remove the ghost peak problem caused by various impurities, saving valuable time and energy for laboratory personnel in drug analysis.
Effective prevention and removal of ghost peak interference in common chromatographic mobile phase systems
Case 1
Figure 1 Water Acetonitrile System（blueTo add the chromatogram after the capture column,redFor unconnected capture columns)
Chromatographic column: Venusil®XBP C18(L) (5μm,4.6*250 mm)
Mobile phase: A: Water, B: acetonitrile
Flow rate: 1.0ml/min
Detection wavelength: UV210nm
Gradient:
Time, min |
Mobile phase A,% |
Mobile phase B,% |
0 |
90 |
10 |
20 |
10 |
90 |
30 |
10 |
90 |
30.1 |
90 |
10 |
45 |
90 |
10 |
Case 2
Figure 2 Mobile phase system containing ammonium acetate（blackTo use the capture column spectrum,Purple;For uncaptured column spectra)
Chromatographic column: Venusil®XBP C18-plus (5μm,4.6*250 mm)
Mobile phase: A: 20mmol/L ammonium acetate (pH 4.0) B: acetonitrile
Flow rate: 1.0ml/min
Detection wavelength: UV280nm
Time, min |
Mobile phase A,% |
Mobile phase B,% |
0 |
95 |
5 |
10 |
80 |
20 |
20 |
40 |
60 |
35 |
20 |
80 |
40 |
20 |
80 |
41 |
95 |
5 |
55 |
95 |
5 |
Case 3
The mobile phase of phosphate system has a higher probability of baseline interference and ghost peaks due to the fluctuation of UV absorption during gradient elution caused by the purity of inorganic salts. We chose a high concentration phosphate system with a large gradient and found that after connecting the ghost peak capture column at the outlet of the mobile phase, the ghost peak was basically eliminated;
Figure 3 Mobile phase system containing sodium dihydrogen phosphate（blueAfter installing the Ghost Hunter capture column,redColor as unconnected Ghost Hunter capture column）
Chromatographic column: Venusil®XBP C18(L) (5μm,4.6*100 mm)
Mobile phase: A: 50mmol/L sodium dihydrogen phosphate pH=4.3, B: acetonitrile
Flow rate: 1.0ml/min
Detection wavelength: UV280nm
Gradient:
Time, min |
Mobile phase A,% |
Mobile phase B,% |
0 |
95 |
5 |
5 |
40 |
60 |
10 |
20 |
80 |
15 |
20 |
80 |
15.1 |
95 |
5 |
25 |
95 |
5 |
ordering information
Product Name |
Specifications |
packing |
Order number |
Ghost Hunter Column Ghost Peak Capture Column |
4.6×50mm |
1/pk |
GHC0505-0 |
matters needing attention
1. The capture column should be stored in methanol before leaving the factory. If phosphate or other salt systems are used, please use an aqueous phase equilibrium transition before use;
If ion pair reagents are added to the mobile phase, the ghost peak capture column may adsorb them, thereby weakening the effect of ions on the reagents and affecting the retention and peak shape of the target substance;
4. The capture column is a consumable, and its actual service life is related to chromatographic conditions, solvent purity, etc. If the capture effect deteriorates, it is recommended to replace it in a timely manner.
5. When connecting the capture column to the liquid phase instrument, it is recommended to control the locking force of the Ruhr connector. If the force is too strong, it may cause jamming. It is recommended to use a two-way interface to connect the capture column and instrument to effectively avoid jamming.